Software for evaluating data derived from native gel electrophoresis of proteins, DNA, viruses, vesicles, conjugated vaccines, etc. Analysis is based on linear or nonlinear Ferguson plots [plot of log(mobility) vs. gel concentration].
Calculations use a physical model rather than empirical polynomial functions. A Newton-Gauss Marquardt-Levenberg curve-fitting algorithm and a mathematical approach based on the "random space walk of a particle through a fiber network" (extended Ogston model) is employed. The program is designed for scientific purposes; it was not the aim to create a 100% Macintosh-like interface. Only a few Mac features have been installed.
ElphoFit is freeware and was developed by Dietmar Tietz at the National Institutes of Health (NIH), Bethesda, Maryland, USA. The program is user-friendly and provides lots of helpful comments.
Additional information on theory, rationales, features, data in/output and graphics is provided in the literature of this Web site and in the Readme files of the ElphoFit software package. Please address suggestions and further questions to:
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