Dedicated to the memory of John S. Fawcett (+ February 2, 1998), an outstanding human being, a highly creative scientist, a good friend and a very helpful colleague

[Manual Book Cover] Information as posted on the Web site of Springer Verlag....

Dietmar Tietz (Ed.)

Nucleic Acid Electrophoresis

Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.

Keywords: Electrophoresis, DNA, forensic science, DNA/protein interaction, analytical biochemistry

Biochemistry; Biotechnology; Human Genetics Written for: Research scientists, advanced students, laboratory technicians

Lab Manual Series
Springer Verlag (Berlin - Heidelberg - New York)
1998, 328 pp., 62 figs., 10 tabs.
ISBN 3-540-63959-4

Table of Contents     Ordering Information

Table of Contents

Nancy Stellwagen (University of Iowa)
DNA Gel Electrophoresis

Alexander Kraev (Swiss Federal Institute of Technology, ETH)
Denaturing Electrophoresis in DNA Sequencing: A Brief History and Current Protocols

Rod Snowdon and Andreas Langsdorf (University of Giessen, Germany)
An Introduction to DNA Fingerprinting USING RFLP and RAPD Techniques

Michael M. Coulthart, Wendy M. Johnson, Fraser E. Ashton (Laboratory Centre for Disease Control, Ontario)
Pulsed-Field Gel Electrophoresis for Genomic fingerprinting of Pathogenic Bacteria

Carl R. Merril, Karen M. Washart (National Institutes of Health, Maryland),
Robert C. Allen (University of South Carolina)
Ultrasensitive Silver Based Stains for Nucleic Acid Detection

Shirin S. Joseph (The Sanger Centre, Cambridgeshire)
The Analysis of Microsatellites by Silver Staining of Nondenaturing Polyacrylamide Gels

John M. Butler and Dennis J. Reeder (National Institutes of Standards, Maryland)
Capillary Electrophoretic Separation of DNA Fragments

Sameer A. Sakallah (University of Pittsburgh, Pennsylvania),
Robert W. Lanning (Carolina Medical Center, North Carolina),
David L. Cooper (Quest Diagnostics, Inc., California)
Rapid Detection of Hepatitis C Virus in Plasma and Liver Biopsies by Capillary Electrophoresis

Hildegard Haas-Rochholz (University of Giessen, Germany)
Electrophoresis in the Field of Forensic Science

Michael G. Fried (Pennsylvania State University),
Mark M. Garner (FMC BioProducts, Maine)
The Electrophoretic Mobility Shift Assay (EMSA) for Detection and Analysis of Protein-DNA Interactions

Jun Xian and Michael G. Harrington (California Institute of Technology)
Mobility shift Analysis of Protein-DNA Complexes by Capillary Electrophoresis

Andreas Kyas, Winfried Mäueler, Joerg T. Epplen (Ruhr-University, Germany)
Polyacrylamide Gel Electrophoresis of DNA/Protein Complexes

David Wheeler (National Institutes of Health, Maryland)
Detection of DNA Curvature Using Transverse Pore Gradient Polyacrylamide Gel Electrophoresis 

 

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