Fig.3: Distribution of signal energy (relative density) in enhanced original (left Panel) and transformed computer images (right Panel). Two-dimensional agarose gel patterns (Serwer-type) are interpreted. The images have been generated by program GelFit. Three representative types of polysaccharide- protein conjugates (I to III) prepared as semi-synthetic meningitis vaccines are shown and compared with two size standards (S). For purposes of a better visualization, pseudocolors are assigned to 16 classes of grey levels. Values of signal energy are expressed as integer numbers from 0 to 255. The ranges for the different colors are as indicated by the calibrated gray scale (bottom left). Left Panel: The horizontal and vertical axis of original images represent migration distances which are non-linearly related to particle size (increasing from right to left) and particle free mobility (increasing from top to bottom). The photos depict a section of the gel with original size of is 14 by 25, 28 by 43, 36 by 32 and 14 by 19 mm for the samples I, II, III, and S, respectively. Right Panel: Images have been transformed from a curvi-linear to rectangular linear coordinate system representing effective values of particle radius and free mobility. Program NIH-IMAGE [by Wayne S. Rasband, NIMH, NIH (email: wayne@helix.nih.gov)] has been used for the composition and labeling of the single images.